Organoid models – 3D culture

Lung organoids provide a model system to study processes of repair/regeneration of lung epithelium. Defective alveolar repair as observed in COPD or lung fibrosis can be recapitulated using this model. Organoids of alveolar epithelial cells in co-culture with fibroblasts and/or endothelial cells can be used to evaluate efficacy of your investigational compounds on stem/progenitor cell functions in the lung.
Contact us today to see how we generate organoids and how your compound can have an effect on them.

TGF-β signalling induces myofibroblast differentiation, which contributes to remodelling in COPD and IPF. We have shown that pre-treatment of human lung fibroblasts (MRC-5 and primary) with TGF-β impairs the ability to support organoid formation. The reduced organoid growth can be rescued by investigational compounds.

Cigarette smoke is one of the main drivers of COPD and contributes to remodeling in COPD. Exposure of organoids to increasing concentrations of cigarette smoke extract reduces the organoid formation with a significant reduction in the number of organoids that are formed per well.

Next to cigarette smoke, exposure to air pollution including diesel particles as in important contributor to COPD. In the organoid model, diesel exposure reduces the organoid formation, comparable to that observed with cigarette smoke exposure.

Furthermore, we have experience with additional stimuli including IL-1B, TNF-a, IL-6, IL-8 and LPS. We continuously improve our organoid models, please reach out to discuss the latest developments.

  • Organoid number
  • Organoid size
  • Differentiation into mature type I and II cells using immunofluorescence or flow cytometry
  • Gene expression patterns. See publication
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